WILD TYPE p53 PROTEIN LEVEL AND MICRONUCLEI NUMBER FORMED AS A BIOMARKER OCCUPATIONAL EXPOSURE OF METAL IN DENTAL TECHNICIANS IN SURABAYA

Titiek Berniyanti, Retno Pudji Rahayu, Indah Listiana Kriswandini

Abstract


Exposure of occupational metals (Co,Ni and Cr) lead to the formation of ROS, and cause damage to DNA. ROS also cause G-T transversion mutation of p53 gene, and lead to low expression of the wild type p53. Wild type p53 plays an important role in regulating genes in cell cycle, cell growth, and DNA repair. If DNA repair is hampered there will be chromosomal damage, thereby effecting the formation of micronuclei. The aim of this study was to analyze the effects of PPE, on wild type p53 and the level of micronuclei in dental technicians. This study was observational analytic with cross sectional approach. 40 samples were taken by random sampling. Data retrieved through interviews and observations. Wild type p53 from saliva was analized with indirect ELISA and micronuclei was examined by bucal swab, then analised by HPA and stained by HE. Data was analised by Pearson correlation test. Significant value is p <0.05.There was a significant correlation between the use of PPE with wild type p53 and micronuclei level (r 0.436 and r -0,337). The study proved that the use PPE properly is positively correlated with the wild p53 and p53 correlated with micronuclei in dental technicians.

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